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The results indicated that CASC2 upregulation contributes significantly to the exercise of Wnt/β-catenin signaling pathway, whereas miR-183 partially reversed the promotion (Fig. Hubei Fuxing Science And Technology Co LtdHubei Fuxing Science And Technology Co Ltdhubei fuxing science and technology co ltdlaw firm HanchuanLaw Firm Hanchuan
In conclusion, CASC2 repressed cell viability, colony formation, migration, and invasion of HCC cells by sponging miR-183. Western blot assay was carried out to analyze protein levels of C-myc, cyclinD, survivin, and β-catenin in SMMC-7721 and Huh-7 cells. To decide the mechanism of CASC2 within the suppression on Wnt/β-catenin signaling pathway, we constructed SMMC-7721 and Huh-7 cells overexpressing CASC2 with or with out miR-183 upregulation. CHIR99021, an agonist of the Wnt/β-catenin pathway, was added in CASC2-overexpressed cells as a optimistic control. To explore the expression of miR-183 in HCC tissues and cell strains, we carried out RT-qPCR assa
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